CoMMiTMenT will develop a novel tool for the detection, selection and complex characterisation of the vulnerable sub-population of RBCs in the blood of patients with RA. This objective will be performed using the novel technique of Optofluidic Microscopy-based Cell Sorting (OMiCS), which is based on fluorescent molecular biomarkers (e.g., antibodies or fluorophores) secondary cellular characteristics, such as the cell shape or other alterations of cellular morphology. By utilising a combination of microfluidics and optical manipulation, positively identified cells can be placed on an adhesive substrate, after which the surface topology of the cell will be determined using Scanning Ion Conductance Microscopy (SICM).
The combination of these imaging approaches will lead to the development of an integrated device, the Combined OMiCS and SIC Microscope (µCOSMOS), where the “µ“ indicates the scale of cellular dimensions. Specifically, the target imaging resolution is at the nanometre level!